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packaging construct pspax2  (Addgene inc)


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    Structured Review

    Addgene inc packaging construct pspax2
    Packaging Construct Pspax2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 15719 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pspax2+packaging+constructs/psPAX2+(Plasmid+%2312260)/pmc13659691-39-11-14
    Average 98 stars, based on 15719 article reviews
    packaging construct pspax2 - by Bioz Stars, 2026-11
    98/100 stars

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    Related Articles

    Produced:

    Article Title: HNF1α maintains pancreatic α and β cell functions in primary human islets.
    Article Snippet: Lentiviral constructs for shRNA targeting exon 4 of HNF1A were obtained from Dharmacon. pLenti-CMV-HNF1A-cMyc-DDK was used in CUT&RUN experiments (OriGene RC211201L1). .. Lentiviruses were produced by transfection of HEK293T cells with lentiviral constructs and pMD2.G and psPAX2 packaging constructs (Addgene). .. TurboFect reagents were used for transfection (Thermo Fisher Scientific), and supernatants were purified using PEG-it (System Biosciences).

    Article Title: Hepatic Transthyretin knockdown alleviates NAFLD by enhancing SERCA2 function and inhibiting endoplasmic reticulum stress
    Article Snippet: .. Then, lentiviruses were produced by transfection of HEK293T cells with lentiviral constructs and pMD2.G and psPAX2 packaging constructs (Addgene). .. PolyJet reagents (Signagen) were used for transfection and the supernatants containing the lentiviruses were centrifuged and filtered, and then stored at -80 °C.

    Article Title: HNF1α transcriptional activation and repression maintain human islet α and β cell function
    Article Snippet: Lentiviral constructs coding for shRNA targeting exon 4 of human HNF1A were obtained from Dharmacon. plenti-CMV-HNF1A-cMyc-DDK was used in CUT&RUN experiments (OriGene Technologies RC211201L1). .. Lentiviruses were produced by transient transfection of HEK293T cells with lentiviral constructs, and with pMD2.G and psPAX2 packaging constructs (Addgene 12259 & 12260). .. TurboFect reagents were used for transfection (Thermo Scientific R0531).

    Article Title: HNF1 α maintains pancreatic α and β cell functions in primary human islets
    Article Snippet: Lentiviral constructs for shRNA targeting exon 4 of HNF1A were obtained from Dharmacon. pLenti-CMV-HNF1A-cMyc-DDK was used in CUT&RUN experiments (OriGene RC211201L1). .. Lentiviruses were produced by transfection of HEK293T cells with lentiviral constructs and pMD2.G and psPAX2 packaging constructs (Addgene). .. TurboFect reagents were used for transfection (Thermo Fisher Scientific), and supernatants were purified using PEG-it (System Biosciences).

    Transfection:

    Article Title: HNF1α maintains pancreatic α and β cell functions in primary human islets.
    Article Snippet: Lentiviral constructs for shRNA targeting exon 4 of HNF1A were obtained from Dharmacon. pLenti-CMV-HNF1A-cMyc-DDK was used in CUT&RUN experiments (OriGene RC211201L1). .. Lentiviruses were produced by transfection of HEK293T cells with lentiviral constructs and pMD2.G and psPAX2 packaging constructs (Addgene). .. TurboFect reagents were used for transfection (Thermo Fisher Scientific), and supernatants were purified using PEG-it (System Biosciences).

    Article Title: Hepatic Transthyretin knockdown alleviates NAFLD by enhancing SERCA2 function and inhibiting endoplasmic reticulum stress
    Article Snippet: .. Then, lentiviruses were produced by transfection of HEK293T cells with lentiviral constructs and pMD2.G and psPAX2 packaging constructs (Addgene). .. PolyJet reagents (Signagen) were used for transfection and the supernatants containing the lentiviruses were centrifuged and filtered, and then stored at -80 °C.

    Article Title: HNF1α transcriptional activation and repression maintain human islet α and β cell function
    Article Snippet: Lentiviral constructs coding for shRNA targeting exon 4 of human HNF1A were obtained from Dharmacon. plenti-CMV-HNF1A-cMyc-DDK was used in CUT&RUN experiments (OriGene Technologies RC211201L1). .. Lentiviruses were produced by transient transfection of HEK293T cells with lentiviral constructs, and with pMD2.G and psPAX2 packaging constructs (Addgene 12259 & 12260). .. TurboFect reagents were used for transfection (Thermo Scientific R0531).

    Article Title: HNF1 α maintains pancreatic α and β cell functions in primary human islets
    Article Snippet: Lentiviral constructs for shRNA targeting exon 4 of HNF1A were obtained from Dharmacon. pLenti-CMV-HNF1A-cMyc-DDK was used in CUT&RUN experiments (OriGene RC211201L1). .. Lentiviruses were produced by transfection of HEK293T cells with lentiviral constructs and pMD2.G and psPAX2 packaging constructs (Addgene). .. TurboFect reagents were used for transfection (Thermo Fisher Scientific), and supernatants were purified using PEG-it (System Biosciences).

    Construct:

    Article Title: HNF1α maintains pancreatic α and β cell functions in primary human islets.
    Article Snippet: Lentiviral constructs for shRNA targeting exon 4 of HNF1A were obtained from Dharmacon. pLenti-CMV-HNF1A-cMyc-DDK was used in CUT&RUN experiments (OriGene RC211201L1). .. Lentiviruses were produced by transfection of HEK293T cells with lentiviral constructs and pMD2.G and psPAX2 packaging constructs (Addgene). .. TurboFect reagents were used for transfection (Thermo Fisher Scientific), and supernatants were purified using PEG-it (System Biosciences).

    Article Title: Hepatic Transthyretin knockdown alleviates NAFLD by enhancing SERCA2 function and inhibiting endoplasmic reticulum stress
    Article Snippet: .. Then, lentiviruses were produced by transfection of HEK293T cells with lentiviral constructs and pMD2.G and psPAX2 packaging constructs (Addgene). .. PolyJet reagents (Signagen) were used for transfection and the supernatants containing the lentiviruses were centrifuged and filtered, and then stored at -80 °C.

    Article Title: HNF1α transcriptional activation and repression maintain human islet α and β cell function
    Article Snippet: Lentiviral constructs coding for shRNA targeting exon 4 of human HNF1A were obtained from Dharmacon. plenti-CMV-HNF1A-cMyc-DDK was used in CUT&RUN experiments (OriGene Technologies RC211201L1). .. Lentiviruses were produced by transient transfection of HEK293T cells with lentiviral constructs, and with pMD2.G and psPAX2 packaging constructs (Addgene 12259 & 12260). .. TurboFect reagents were used for transfection (Thermo Scientific R0531).

    Article Title: HNF1 α maintains pancreatic α and β cell functions in primary human islets
    Article Snippet: Lentiviral constructs for shRNA targeting exon 4 of HNF1A were obtained from Dharmacon. pLenti-CMV-HNF1A-cMyc-DDK was used in CUT&RUN experiments (OriGene RC211201L1). .. Lentiviruses were produced by transfection of HEK293T cells with lentiviral constructs and pMD2.G and psPAX2 packaging constructs (Addgene). .. TurboFect reagents were used for transfection (Thermo Fisher Scientific), and supernatants were purified using PEG-it (System Biosciences).



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